Comparative analyses of molecular parameters inducing cellular and clinical radiosensitivity - 3604S04439
urn:nbn:de:0221-2009100625 BfS-RESFOR-23/09
Summary
Within the research project primary blood cells of approx. 100 patients each from 2 different clinical cohorts were analyzed with respect to cellular sensitivity to ionising radiation. Using the Comet assay and FASC-based apoptosis assays, the DNA-repair capacity and cellular sensitivity upon irradiation was detected. Both parameters detected conspicuous samples of altered radiosensitivity. In EBV-immortalized derivatives of such samples, molecular analyses detected novel mutations in candidate genes for radiosensitivity. For the conspicuous samples, no clear correlation was evident between cellular or molecular phenotypes and clinical radiosensitivity. In individual cases, however, the detection of novel mutations in candidate genes suggests that clinical tumorigenesis and / or clinical radiosensitivity may be due to genetic predisposition.
